Expression of the <i>soxRS</i> regulon in bacterial cells exposed to various stress factors

نویسندگان

چکیده

Background. Some stress responses contribute to the formation of bacterial antibiotic resistance, including soxRS oxidative defense regulon. Elevation reactive oxygen species production and was detected in cells exposed various environmental stresses. It can be supposed that a stress-mediated increase level will activate expression regulon genes, which may provide pre-adaptation antibiotics. The aim. To study changes genes Escherichia coli NaCl, acetic acid, heating. Materials methods . Gene measured bearing reporter gene fusions (soxS::lacZ, nfo::lacZ). An overnight broth culture diluted fresh LB OD600 = 0.1 cultivated at 37 °C without stirring until 0.3, then stressors were applied. Results. Exposure NaCl acid activated while heating caused decrease expression. observed subjected stresses low intensity (which did not cause number colony-forming units (CFU) by 4 th hour exposure compared beginning exposure) medium 10-fold CFU), whereas high-intensity CFU more than 10 times), regardless their nature, accompanied genes. Conclusion. Under conditions studied, only osmotic addition significant activation Sublethal causing an 2–2.5 times, bacteria factors this is aimed counteracting, antibacterial drugs.

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ژورنال

عنوان ژورنال: Acta biomedica scientifica

سال: 2023

ISSN: ['2541-9420', '2587-9596']

DOI: https://doi.org/10.29413/abs.2023-8.2.11